Work

RNA extraction, probe preparation, and competitive hybridization for transcriptional profiling using Neurospora crassa long-oligomer DNA microarrays

Year: 2008

Type: article

Abstract: We developed protocols optimized for the performance of experiments assaying genomic gene expression using Neurospora crassa long-oligomer microarrays. We present methods for sample growth and harvesting, total RNA extraction, poly(A)+ mRNA selection, preparation of NH3-Allyl Cy3/Cy5 labeled probes, and microarray hybridization. The quality of the data obtained with these protocols is demonstrated by the comparative transcriptional profiling of basal and apical zones of vegetative growth of N. crassa.

Source: Fungal Genetics Reports

DOI: 10.4148/1941-4765.1086

Authors: Travis A. Clark, Julie M. Guilmette, Daniel Renstrom, Jeffrey P. Townsend

Institutions: Yale University, University of Connecticut

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: National Institutes of Health

Award: G9000053803