Year: 2011
Type: article
Abstract: The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.
Source: Small
Institutions: Université Paris-Sud, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, École Normale Supérieure Paris-Saclay, Laboratoire de Photonique Quantique et Moléculaire, École Polytechnique, Laboratoire de Physique de la Matière Condensée, École Normale Supérieure - PSL, Laboratoire des Biomolécules, Université Pierre-et-Marie-Curie
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2594430330