Work

Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

Year: 2017

Type: book-chapter

Abstract: Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.

Source: Methods in molecular biology

DOI: 10.1007/978-1-4939-6771-1_11

Authors: Matthew Tierney, Alessandra Sacco

Institution: Sanford Burnham Prebys Medical Discovery Institute

Language: English

Topic: Muscle Physiology and Disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: Medical University of South Carolina, National Institute of Arthritis and Musculoskeletal and Skin Diseases