Work

Selective single cell detachment and retrieval for downstream analyses using nanosecond laser pulses in cnt-coated microwell arrays

Year: 2015

Type: conference-paper

Abstract: Cellular heterogeneity is one of the key hallmarks in cancer biology, but conventional dish-based assays only report the average behavior of many cells. Microfluidics can facilitate manipulating and monitoring of individual cells, yet it is difficult to retrieve a specific target single cell from an enclosed microfluidic chip. In this work, we have successfully developed a selective cell detachment and retrieval scheme with a spatial resolution of around 10μm. The retrieved cells were proved to be viable, and the detachment process has negligible effect on membrane proteins and mRNA expression, providing an ideal tools for the downstream analysis of target cells.

Source: DSpace@MIT (Massachusetts Institute of Technology)

Authors: Yu Chih Chen, Hyoung Won Baac, Kyu Tae Lee, Kendall Teichert, A. John Hart, L. Jay Guo, Euisik Yoon

Institutions: University of Michigan, Sungkyunkwan University, Massachusetts Institute of Technology

Language: English

Topic: Microfluidic and Bio-sensing Technologies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funder: U.S. Department of Defense

Awards: G4119534169, PERSONALIZED MEDICINE TARGETING OVARIAN CANCER-INTIATING CELLS

OpenAlex ID: W2595563421

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