Work

Diffuse fluorescence fiber probe for in vivo detection of circulating cells

Year: 2017

Type: article

Abstract: There has been significant recent interest in the development of technologies for enumeration of rare circulating cells directly in the bloodstream in many areas of research, for example, in small animal models of circulating tumor cell dissemination during cancer metastasis. We describe a fiber-based optical probe that allows fluorescence detection of labeled circulating cells in vivo in a diffuse reflectance configuration. We validated this probe in a tissue-mimicking flow phantom model in vitro and in nude mice injected with fluorescently labeled multiple myeloma cells in vivo. Compared to our previous work, this design yields an improvement in detection signal-to-noise ratio of 10 dB, virtually eliminates problematic motion artifacts due to mouse breathing, and potentially allows operation in larger animals and limbs.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.jbo.22.3.037004

Authors: Vivian E. Pera, Xuefei Tan, Judith M. Runnels, Neha R. Sardesai, Charles P. Lin, Mark Niedre