Work

Wide-field imaging combined with confocal microscopy using a miniature f/5 camera integrated within a high NA objective lens

Year: 2017

Type: article

Abstract: Wide-field (WF) imaging paired with reflectance confocal microscopy can noninvasively detect skin cancer with high accuracy. However, two separate devices are required to perform each imaging procedure. We describe a new concept that integrates the two into one device: a miniature WF color camera within the objective lens used for confocal microscopy, providing simultaneous sub-surface cellular imaging and WF surface morphologic imaging. The camera, inserted between a hyperhemisphere front lens and a back lens group of the objective, commands a field of view of 4.0 mm, with a resolution better than 30 μm, while confocal optical sectioning is preserved at sharper than 2.5 μm.

Source: Optics Letters

DOI: 10.1364/ol.42.001241

Authors: David L. Dickensheets, Seth Kreitinger, Gary Peterson, Michael Heger, Milind Rajadhyaksha

Institutions: Montana State University, Memorial Sloan Kettering Cancer Center

Language: English

Topic: Advanced optical system design

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Directorate for Engineering, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering