Work

Dopamine-modified TiO 2 monolith-assisted LDI MS imaging for simultaneous localization of small metabolites and lipids in mouse brain tissue with enhanced detection selectivity and sensitivity

Year: 2017

Type: article

Abstract: -DA monolith-assisted LDI MSI approach has higher selectivity and sensitivity for Lewis basic compounds, such as fatty acids, cholesterols, ceramides, diacylglycerols, and phosphatidylethanolamine, when analyzed in positive mode, than traditional MALDI MS. Using this new method, over 100 molecules, including amino acids, alkaloids, free fatty acids, peptides, and lipids, were localized in mouse brain sections. By comparing the presence and localization of those molecules in young and old mouse brains, the approach demonstrated good performance in the determination of aging-related neurochemical changes in the brain.

Source: Chemical Science

DOI: 10.1039/c7sc00937b

Authors: Qian Wu, James L. Chu, Stanislav S. Rubakhin, Martha U. Gillette, Jonathan V. Sweedler

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: University of Illinois at Urbana-Champaign, National Institute on Drug Abuse