Year: 2016
Type: conference-paper
Abstract: Results and discussionThe ability to self-assemble to form hydrogels is determined by the primary peptide structure.Naproxen and indomethacin conjugated peptides formed self-supporting hydrogels.Ibuprofen conjugates due to insufficient π-π stacking, remained as viscous solutions confirmed via viscosity measurements.Rheological analysis confirmed the hydrogels as viscoelastic with G' being one order of magnitude higher than G'' for all concentrations of peptides.FTIR further confirmed assembly with shoulders in the amide I region (1570 cm -1 ) and characteristic peaks representative of β-sheet formation (1625 cm -1 ) in an antiparallel arrangement (1675 cm -1 ).Higher ordered nanofibrous assemblies were observed under cryo-SEM and TEM.The hydrogels proved to be relatively non-hemolytic and non-toxic in vitro.Strong antibiofilm activity was demonstrated by all hydrogels.The optimal reduction in percentage viable biofilm was exhibited by IndFFKK against Staphylococcus epidermidis.The anti-inflammatory activity of the NSAIDs was not inhibited following coupling to the peptide with hydrogels showing inhibitory activity against both COX-1 and COX-2 enzymes.
Authors: Alice McCloskey, Sophie Gilmore, Simon Porter, Brendan Gilmore, Garry Laverty
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2599738329