Year: 2016
Type: dissertation
Abstract: Natural pentatricopeptide repeat proteins are mostly insoluble and bind RNA in an unpredictable manner. We have designed a consensus PPR (cPPR) that is programmable, more stable and soluble. The cPPR binds to specific RNA depending on the choice of amino acids at position 4 and 34 of each cPPR repeat. I showed that cPPRs can be re-designed to block mitochondrial protein translation and microRNA. Interestingly cPPRs can also bind single-stranded DNA and regulate human telomerase in vitro. This is the first study on re-designing a natural RNA-binding protein to target ssDNA, mRNA and microRNA.
Author: Tiong Sun Chia
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2599925048