Year: 2012
Type: article
Abstract: The subject of this dissertation is the development of mass spectrometry-based methods for the discovery of proteomic biomarkers.Two different projects are presented herein.Chapters 2-4 describe the discovery of protein biomarkers for early detection and/or to monitor bladder cancer recurrence, progression, and response to treatment by the use of mass spectrometry-based proteomics.Chapter 5 describes a mass spectrometry workflow for the identification of therapeutic biomarkers associated with Silvestrol treatment in chronic lymphocytic leukemia (CLL).Chapter 2 focuses on the development of histone H1 phosphorylation as a diagnostic and prognostic biomarker.The post-translational modifications of histones extracted from bladder cancer cells were analyzed by liquid chromatography mass spectrometry (LC-MS).Increased phosphorylation of the H1 histones in invasive (J82, T24 and UMUC3) compared to non-invasive (RT4) bladder cancer cell lines and cultured epithelial cells was discovered.The epithelial cells demonstrated the least histone phosphorylation compared to all others.Immunohistochemistry (IHC) was performed on one replicate of patient tissue samples to determine if H1 phosphorylation can indicate invasive cells in human tissues and preliminary results are positive.IHC results showed increased H1 phosphorylation in bladder cancer tissues compared to normal urothelium.
Author: Kelly H. Telu
Institution: The Ohio State University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2601580166