Investigation of a relationship between the core PAT family proteins and their expression in adipose tissue from specific depots of three mouse models with varying levels of GH signaling
Year: 2007
Type: article
Abstract: A better understanding of the storage and release of neutral lipids from adipocytes is vital to understanding obesity and diabetes.Work in recent years has revealed a central role for perilipin, a lipid droplet surface protein, for optimal triacylglycerol storage and fatty acid release.Perilipin belongs to the PAT family of proteins, including perilipin, ADRP, and TIP47.Perilipin and ADRP are most closely related and are responsible for controlling lipase access to TAG.Growth hormone (GH) has repeatedly been shown to induce lipolysis and inhibit lipogenesis although the mechanism is not well understood.The potential relationship between GH and the PAT family has yet to be examined.To address this, we utilized two mouse lines with altered GH signaling: 1) GH receptor gene disrupted (GHR -/-) mice; and 2) mice expressing bovine GH (bGH).Perilipin, ADRP, and TIP47 mRNA expression was compared in three adipose depots (subcutaneous, epididymal and retroperitoneal) in GHR -/-, bGH, and wild type (WT) mice on a high fat (HF) or low fat (LF) diet using quantitative real time PCR.Results showed a significant decrease in perilipin mRNA in the subcutaneous and retroperitoneal depots of bGH mice on the LF diet compared to GHR -/-and WT mice.However, there was no significant difference in perilipin mRNA expression between the GHR -/-and WT mice in any of the iv fat depots.ADRP mRNA expression levels are more variable than perilipin mRNA levels with respect to genotype and diet treatment.This research may provide insight into the relationship between GH and the lipid packaging functions of the PAT family and may aid in discovering a prospective target to aid in the treatment of obesity.
Author: Stacy L Kolbash
Institution: Ohio University
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2601942897