Work

Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells

Year: 2017

Type: article

Abstract: Efficient delivery of antigens is of paramount concern in immunotherapies. We aimed to target antigen presenting cells (APCs) by conjugating CpG oligonucleotides to an E2 protein nanoparticle surface (CpG-PEG-E2). Compared to E2 alone, we observed ∼4-fold increase of in vitro APC uptake of both CpG-PEG-E2 and E2 conjugated to non-CpG DNA. Furthermore, compared to E2-alone or E2 functionalized solely with polyethylene glycol (PEG), the CpG-PEG-E2 showed enhanced lymph node retention up to at least 48 h and 2-fold increase in APC uptake in vivo, parameters which are advantageous for vaccine success. This suggests that enhanced APC uptake of nanoparticles mediated by oligonucleotide display may help overcome delivery barriers in vaccine development.

Source: ACS Biomaterials Science & Engineering

DOI: 10.1021/acsbiomaterials.7b00148

Authors: Nicholas M. Molino, Medea Neek, Jo Anne Tucker, Edward L. Nelson, Szu-Wen Wang

Institution: University of California, Irvine

Language: English

Topic: Immunotherapy and Immune Responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Cancer Research Coordinating Committee, National Cancer Institute, National Institute of Biomedical Imaging and Bioengineering