Work

Sucrose sensing through nascent peptide‐meditated ribosome stalling at the stop codon of Arabidopsis bZIP 11 uORF 2

Year: 2017

Type: article

Abstract: Arabidopsis bZIP11 is a transcription factor that modulates amino acid metabolism under high-sucrose conditions. Expression of bZIP11 is downregulated in a sucrose-dependent manner during translation. Previous in vivo studies have identified the second upstream open reading frame (uORF2) as an essential regulatory element for the sucrose-dependent translational repression of bZIP11. However, it remains unclear how uORF2 represses bZIP11 expression under high-sucrose conditions. Through biochemical experiments using cell-free translation systems, we report on sucrose-mediated ribosome stalling at the stop codon of uORF2. The C-terminal 10 amino acids (29-SFSVxFLxxLYYV-41) of uORF2 are important for ribosome stalling. Our results demonstrate that uORF2 encodes a regulatory nascent peptide that functions to sense intracellular sucrose abundance. This is the first biochemical identification of the intracellular sucrose sensor.

Source: FEBS Letters

DOI: 10.1002/1873-3468.12634

Authors: Yui Yamashita, Seidai Takamatsu, Michael Glasbrenner, Thomas Becker, Satoshi Naito, Roland Beckmann

Institutions: Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universität München, Hokkaido University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Deutsche Forschungsgemeinschaft, Ministry of Education, Culture, Sports, Science and Technology, European Research Council, Japan Society for the Promotion of Science

Awards: G1857377308, G4104527358, G5598557700