Work

Association of Tomato Yellow Leaf Curl Virus and Chili Leaf Curl Virus with Leaf Curl Disease of Radish and the Synergistic Interaction on Nicotiana benthamiana

Year: 2017

Type: article

Abstract: Leaf yellowing and rolling with slight crinkling was observed in radish plants in Oman, indicative of a begomovirus infection.Viral sequences from radish were 99% identical to sequences of Tomato yellow leaf curl virus (TYLCV) and Chilli leaf curl virus (ChiLCV), from Oman.No beta-or alpha-satellites were detected.Infectious clones were developed and infectivity analyses of isolated viruses were done in a model host Nicotiana benthamiana.Both viruses, when inoculated separately, produced typical symptoms; however, symptom severity increased when these viruses were co-inoculated.Southern analyses confirmed that virus accumulation was high when both viruses were inoculated as compared to independent inoculation.Realtime qPCR analyses showed that copy number of both viruses was 1 X 10 6 copies when N. benthamiana plants were inoculated with either TYLCV or ChiLCV; however, the copy number increased to 1 X 10 8 copies when the two viruses were co-inoculated together in N. benthamiana.

Source: International Journal of Agriculture and Biology

DOI: 10.17957/ijab/15.0273

Authors: Adel A. Al-Shihi, Um e Ammara, Imran Amin, Michael Deadman, Abdullah M. Al-Sadi

Institutions: Sultan Qaboos University, National Institute for Biotechnology and Genetic Engineering

Language: English

Topic: Plant Virus Research Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funder: Sultan Qaboos University