Work

Mp31-04 the Power of Crowdsourcing: Novel Method for Discovery of Urine Biomarkers

Year: 2017

Type: article

Abstract: quantitative sensory testing, and urine sample collection.Temporal summation to evoked, thermal cutaneous pain was performed with a Medoc Thermal Sensory Analyzer at .4 Hz, a frequency known to elicit C-fiber mediated wind-up in the dorsal horn of the spinal cord.Subjects were asked to rate their pain (0 e 100 VAS) during each of a sequence of 10 brief (.5 second) heat pulses to 49 C. Temporal summation was defined as the difference in pain rating between the maximum and first pain ratings.An individual with a difference in pain ratings or a first pain rating greater than 1 SD above controls after normalization was designated as demonstrating CS.Mid-stream urine samples collected from each patient were subjected to metagenomic sequencing targeting the V3-V4 region of the 16S-rRNA gene.Relative bacterial abundances were compared using the QIIME and the Wald test statistic in the MGLM package among women with and without OAB and CS.RESULTS: 23 patients comprised the study cohort.6/10 (60%) subjects with OAB demonstrated CS, 2/8 (25%) subjects demonstrating CS did not have OAB.Bacterial abundance differed significantly between patients with and without OAB (Wald test statistic 316, p<0.01) and CS (Wald test statistic 80, p<0.01).Relative bacterial abundances were similar in patients with OAB and CS (Table ).Relative to those without OAB and CS, Enterobacteriaceae, Chitinophagaceae and Burkholderiaceae were more abundant in subjects with OAB and CS and Lactobacillaceae and Prevotellaceae less abundant.CONCLUSIONS: C-fiber activation related to elevated CS and alterations in the urinary microbiome may represent a combined mechanism of action for the development of refractory LUTS in some patients that warrants further study.

Source: The Journal of Urology

DOI: 10.1016/j.juro.2017.02.958