Coffee and chlorogenic acid inhibit hydrogen peroxide induced apoptosis in primary cortical neurons
Year: 2013
Type: dissertation
Abstract: Oxidative injury has been linked causally to a variety of neurodegenerative diseases.Oxidative injury is mediated by reactive oxygen species (ROS) such as hydrogen peroxide (H2O2).Epidemiological studies have suggested that coffee may be neuroprotective, but their biological mechanisms are poorly understood.In this study, i investigated the protective effects of caffeinated coffee, decaffeinated coffee, and the phenolic phytochemical chlorogenic acid (5-O-caffeoylquinic acid), which is present in both caffeinated and decaffeinated coffee, against apoptotic cell death.I conformed neuroprotective effect of caffeinated coffee, decaffeinated coffee and chlorogenic acid thorough DAPI staining which identified H2O2-induced apoptotic nuclear condensation in neuronal cells.Caffeinated coffee, decaffeinated coffee, or chlorogenic acid also blocked the H2O2-induced down-regulation of anti-apoptotic proteins Bcl-2 and Bcl-XL while ii blocking H2O2-induced pro-apoptotic cleavage of caspase-3 and pro-poly (ADP-ribose) polymerase.I also found that caffeinated coffee, decaffeinated coffee, and chlorogenic acid induced the expression of NADPH:quinine oxidoreductase 1 (NQO1) in neuronal cells, suggesting that these substances protect neurons from H2O2-induced apoptosis by upregulation of this antioxidant enzyme.The neuroprotective efficacy of caffeinated coffee was similar to that of decaffeinated coffee, indicating that active compounds present in both caffeinated and decaffeinated coffee, such as chlorogenic acid, may drive the effects.
Author: 이시영
Language: English
Topic: Coffee research and impacts
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2606799173