Year: 2017
Type: preprint
Abstract: Mouse embryonic stem cells are derived from in vitro explantation of blastocyst epiblasts 1,2 and contribute to both the somatic lineage and germline when returned to the blastocyst 3 but are normally excluded from the trophoblast lineage and primitive endoderm 4–6 . Here, we report that cultures of expanded potential stem cells (EPSCs) can be established from individual blastomeres, by direct conversion of mouse embryonic stem cells (ESCs) and by genetically reprogramming somatic cells. Remarkably, a single EPSC contributes to the embryo proper and placenta trophoblasts in chimeras. Critically, culturing EPSCs in a trophoblast stem cell (TSC) culture condition permits direct establishment of TSC lines without genetic modification. Molecular analyses including single cell RNA-seq reveal that EPSCs share cardinal pluripotency features with ESCs but have an enriched blastomere transcriptomic signature and a dynamic DNA methylome. These proof-of-concept results open up the possibility of establishing cultures of similar stem cells in other mammalian species.
DOI: 10.1101/124479
Authors: Jian Yang, David J. Ryan, Wei Wang, Jason Cheuk-Ho Tsang, Guocheng Lan, Hideki Masaki, Xuefei Gao, Liliana Antunes, Yong Yu, Zhexin Zhu, Juexuan Wang, Aleksandra A. Kolodziejczyk, Lia S. Campos, Cui Wang, Fengtang Yang, Zhen Zhong, Beiyuan Fu, Melanie Eckersley-Maslin, Michael Woods, Yosuke Tanaka, Adam C. Wilkinson, James Bussell, Jacqui White, Ramiro Ramirez-Solis, Wolf Reik, Berthold Göttgens, Sarah A. Teichmann, Hiromitsu Nakauchi, Xiangang Zou, Liming Lu, Pentao Liu
Institutions: Wellcome Sanger Institute, Chinese University of Hong Kong, Prince of Wales Hospital, University of Cambridge, Cancer Research UK, Cancer Research UK Cambridge Center, Japan Science and Technology Agency, European Bioinformatics Institute, MRC Laboratory of Molecular Biology, Medical Research Council, Babraham Institute, Wellcome/MRC Cambridge Stem Cell Institute, Wellcome Trust, Shanghai Jiao Tong University, Shanghai Institute of Immunology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Wellcome Trust, Cancer Research UK, National Natural Science Foundation of China, Russian Science Foundation, Biotechnology and Biological Sciences Research Council, Japan Society for the Promotion of Science, Fundação para a Ciência e a Tecnologia, Cancer Research UK Cambridge Institute, University of Cambridge
Awards: G1085860639, G1451698671, G2330126045, Adaptation of a high resolution, high throughput assay of immunoglobulin repertoires for commercial and clinical applications, G5503369614, II类反式激活因子通过NLR信号调控Th2应答的分子免疫学机制, Epigenetic reprogramming in mammalian development., G7378106769, 心理学におけるEEGならびGSRに測定法の研究(継2年), G8525317206, Wellcome Trust Sanger Institute - generic account for deposition of all core- funded research papers, G8780693355
OpenAlex ID: W2607055080