Characterization of CbCAX51, a Cold Responsive Ca2+/H+ Exchanger from Capsella bursa-pastoris Modulating Cold Tolerance in Plants
Year: 2017
Type: article
Abstract: The CAX (Calcium Exchanger) genes constitute a set of signal transductors that are important for plant growth and adaptation to environmental stresses, especially to low temperature.CbCAX51, a Ca 2+ /H + exchanger was cloned form Capsella bursa-pastoris, has been reported as a cold inducible gene.Here we showed that the transcriptional level of CbCAX51 in leaf and stem was higher than that in roots.Transcript of CbCAX51 was continuously up-regulated by Ca 2+ and Cu 2+ , and transiently up-regulated by Zn 2+ and Li + , cold stress, GA and ABA.The CbCAX51 protein was predicted to be localized in vacuolar by PSORT to Plant-mPLos, which was further confirmed by the subcellular detection of CbCAX51-GFP fusion protein.When CbCAX51 was transformed into the cold-sensitive plant tobacco, it conferred cold tolerance in tobacco seedlings based on physiological studies.Likewise, overexpression of CbCAX51 can activated some cold responsive genes in transgenic tobacco.Taken together, CbCAX51 is involved in the cold signal transduction and modulation of downstream components to enhance cold tolerance in tobacco.
Author: Weiwei Li
Institution: Fudan University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: bronze
Award: 低温介导的CBF与赤霉素途径互作抑制植物生长的机制研究
OpenAlex ID: W2607445752