Work

Label-free three-dimensional (3D) structural imaging in live cells using intrinsic optical refractive index

Year: 2017

Type: preprint

Abstract: Here we report a method for visualization of volumetric structural information of live biological samples with no exogenous contrast agents. The process is made possible through a technique that involves generation, synthesis and analysis of three-dimensional (3D) Fourier components of light diffracted by the sample. This leads to the direct recovery of quantitative cellular morphology with no iterative procedures for reduced computational complexity. Combing with the fact that the technique is easily adaptive to any imaging platform and requires minimum sample preparation, our proposed method is particularly promising for observing fast, volumetric and dynamic events previously only accessible through staining methods.

Source: arXiv (Cornell University)

DOI: 10.48550/arxiv.1704.04445

Authors: Liu, Chen, Malek, Michael, Poon, Ivan K. H., Jiang, Lanzhou, Sheppard, Colin J. R., Roberts, Ann, Quiney, Harry, Zhang, Douguo, Yuan, Xiaocong, Lin, Jiao, Depeursinge, Christian, Marquet, Pierre, Kou, Shan Shan

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funder: National Natural Science Foundation of China

Award: G3674526924

OpenAlex ID: W2607465793

Cites:

Cited by: 2

Related to: 10