Work

Changes in human hepatic metabolism in steatosis and cirrhosis

Year: 2017

Type: article

Abstract: AIM: To understand the underlying metabolic changes in human liver disease we have applied nuclear magnetic resonance (NMR) metabolomics analysis to human liver tissue. METHODS: H-NMR to derive metabolomic signatures from human liver from patients with steatosis, nonalcoholic steatohepatitis (NASH) or alcohol-related liver damage (ARLD) to identify species that can predict outcome and discriminate between alcohol and metabolic-induced liver injuries. RESULTS: Changes in branched chain amino acid homeostasis, tricarboxylic acid cycle and purine biosynthesis intermediates along with betaine were associated with the development of cirrhosis in both ARLD and nonalcoholic fatty liver disease. Species such as propylene glycol and as yet unidentified moieties that allowed discrimination between NASH and ARLD samples were also detected using our approach. CONCLUSION: Our high throughput, non-destructive technique for multiple analyte quantification in human liver specimens has potential for identification of biomarkers with prognostic and diagnostic significance.

Source: World Journal of Gastroenterology

DOI: 10.3748/wjg.v23.i15.2685

Authors: Zoe Schofield, Michelle AC Reed, Philip N Newsome, David H Adams, Ulrich L Günther, Patricia F Lalor

Institutions: Engineering and Physical Sciences Research Council, University of Birmingham

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: University Hospitals Birmingham NHS Foundation Trust, National Institute for Health and Care Research, University of Birmingham