Effect of Remote Ischemic Conditioning on Lipopolysaccharide-induced Pulmonary Inflammation
Year: 2016
Type: dissertation
Abstract: Remote ischemic preconditioning (RIPC), an intervention whereby an extremity undergoes brief repeated cycles of ischemia/reperfusion (I/R), has been shown to exert protective effects on distant organ I/R injury. Its potential benefit in organ injury induced by other inflammatory stimuli has not been determined. To test whether RIPC would exert protective effects on LPS-induced pulmonary inflammation, mice were subjected to hindlimb RIPC using a tourniquet prior to intratracheal LPS. RIPC significantly reduced leukocyte recruitment and protein leakage in the lungs at 4 hours after LPS. Lung ICAM-1 mRNA expression was also reduced, whereas TNF-ι and IL-1β were upregulated, although these changes were statistically insignificant. However, the protective effect of RIPC was lost by 24 hours after LPS. Moreover, delayed conditioning and dual conditioning before and after LPS administration did not prevent lung inflammation. In conclusion, RIPC provides early protection against LPS-induced pulmonary inflammation in mice, but the effect is not sustained.
Source: TSpace (University of Toronto)
Author: So Jung Choi
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2607596931