P03.14 Validation of microscopy with ultraviolet surface excitation - MUSE - imaging of human primary and metastatic brain tumors
Year: 2017
Type: conference-abstract
Abstract: Introduction: Small tissue specimens, like brain tissue core-needle biopsies, can be consumed during the primary morphological diagnostics, leaving little or no tumor sample available for molecular analyses. The consequence is either that the patient receives no molecular data, or may be subjected to additional, uncomfortable, and occasionally dangerous, brain tissue-harvesting procedures. Development of tissue-sparing, slide-free methods for rapid tissue histological analysis may eliminate a need for frozen section and ensure tissue availability for molecular testing in addition to morphological diagnosis. Materials and Methods: In this pilot study we have validated diagnostic reliability of MUSE (microscopy using ultraviolet surface excitation) imaging – a novel, tissue-sparing microscopy technique that obviates conventional histology processing, formalin-fixation and paraffin-embedding (FFPE) – in the pathologic analysis of primary and metastatic brain and spinal cord tumors. A panel of board-certified pathologists conducted blinded analysis to compare diagnostic accuracy of MUSE images vs. standard FFPE, microtome-sectioned and H&E-stained slides of the same brain tumor specimens. Any differences in diagnosis (either between MUSE and conventional methodology or within the panel of observers) were graded on a scale of (0) equivalent (1) minor difference probably not clinically significant (2) intermediate difference such as differences in size measurements or grade (3) major difference (falsely positive or negative). Results: 24 adult patients (14 male, 10 female), mean age 54 years (range 19 – 83 years), who underwent surgical resection for newly diagnosed brain and spinal cord tumors were included into the study. 7/24 (29%) were diagnosed with diffuse astrocytic or oligodendroglial tumors; 8/24 (33.5%) with meningioma’s; 3/24 (12.5%) with ependymal and choroid plexus tumors; 3/24 (12.5%) with tumors of the cranial or paraspinal nerves and 3/24 (12.5%) with metastatic tumors by the conventional methodology. We have found 97% concordance between MUSE and light microscopy diagnostics across all examined brain tumors, with 94% overall concordance (87% based on tumor grade) within the pathologists panel. In addition, MUSE topographical information from portions of samples below the plane of the cut surface provided novel 2.5-dimensional insights, especially valuable for analyzing complex neuroanatomical structures. Conclusions: Diagnostic validation of MUSE imaging reported here serves as a proof-of-concept showing that this new optical microscopy technique may rapidly generate diagnostic-quality histological images of brain and spinal cord tumors, with high resolution and enhanced content, from fresh or fixed tissue. More extensive studies are thus warranted to fully evaluate potential of using MUSE in routine neuropathology practice.
Source: Neuro-Oncology
Authors: M. Lechpammer, M. Tian, K. Shahlaie, F. Fereidouni, Z. T. Harmany, A. D. Borowsky, D. Zagzag, R. M. Levenson
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2608309030