GABA-A Receptor Complex in the Anxiolytic Properties of Parkia biglobosa in Mice
Year: 2017
Type: article
Abstract: Parkia biglobosa (Mimosaceae) is a plant about 10 to 15 meters high and is present in the Sudano-sahel region. It is used in Cameroon traditional medicine to treat hypertension, joint pain, zona and in osteopathy. The present study was aimed at studying the anxiolytic properties of the aqueous extract of Parkia biglobosa in mice. The pharmacological tests such as tests of Hyperthermia Induced-Stress (HIS), Open field(OF) and elevated plus maze (EPM) were used. Four doses of the plant extract 10; 25; 50 and I00 m/kg were equally used. The value of SIH was 2.27°C in the negative control group and 1.30°C with mice treated by Phenobarbital 20 mg/kg. Treatments of the animals with gradual doses of P. biglobosa led to HIS significant decrease to 1.43°C corresponding to the dose of 100 mg/kg and representing more than 80% of the value obtained by the reduction of body temperature. In the EPM test there was a significant increase in percentage of entries into the open arms from 28.76% for the negative control group to 69.08 and 70.95% for 50 and 100 mg/kg respectively. The diazepam (DZP) 0.3 mg/kg increased this value to 73%. Then, in the test of the benzodiazepine antagonist, it was observed that the percentage of time spent in open arms decreased from 30% in the negative control group to a significant value of 11% in the distilled water and FG7142, treated groups, whereas this percentage increased significantly to 63.3, 66.6 and 50% at doses of Distilled water and 50 mg/kg, Distilled water and +100 mg/kg, and also flumazenil and 100 mg/kg of aqueous extract of plant respectively. Subsequently, Gaboxadol(GAB) agonist and Bicuculine(BIC) competitive antagonist of Gaba receptor sites were used as reference substances of Gaba site of GABA-A receptors complex and this test showed a significant increased in the percentage of time spent in the open arms from 14.54% for the negative control group to 39.11, 43.45 and 33.97% for Distilled water and Gaboxadol, Distilled water and 50 mg and ED+100 mg/kg groups of the aqueous extract of plant respectively, while there was a significant decrease in this percentage to 7.82, 9.49, 9.55 and 6.94% for BIC+ED, BIC+GAB, BIC+50 and BIC+100 mg/kg groups respectively. These results reveal that P. biglobosa showed anxiolytic properties in the mice model tests.
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2608701361