Work

A conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9

Year: 2017

Type: article

Abstract: A conformational checkpoint of Cas9 enables on-target cleavage while blocking access to the active state at off-target sites.

Source: Science Advances

DOI: 10.1126/sciadv.aao0027

Authors: Yavuz S. Dagdas, Janice S. Chen, Samuel H. Sternberg, Jennifer A. Doudna, Ahmet Yildiz

Institutions: University of California, Berkeley, Howard Hughes Medical Institute, Lawrence Berkeley National Laboratory

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $4,500

Funders: National Science Foundation, Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences

Awards: G1048358990, G1728853443, Mechanisms of Acquired Immunity in Bacteria, G4696300853, G4789931749, Understanding the Mechanism and Regulation of the Human Cytoplasmic Dynein Complex, CAREER: Single Molecule Studies to Dissect the Mechanochemistry of Cytoplasmic Dynein, G6438499386, The Role of Shelterin-Mediated Telomere Remodeling in Chromosome End Protection

OpenAlex ID: W2953019923

FWCI: 10.72

Cites: 32

Cited by: 293

Related to: 10