Work

Optimization of fluorescent imaging in the operating room through pulsed acquisition and gating to ambient background cycling

Year: 2017

Type: article

Abstract: The design of fluorescence imaging instruments for surgical guidance is rapidly evolving, and a key issue is to efficiently capture signals with high ambient room lighting. Here, we introduce a novel time-gated approach to fluorescence imaging synchronizing acquisition to the 120 Hz light of the room, with pulsed LED excitation and gated ICCD detection. It is shown that under bright ambient room light this technique allows for the detection of physiologically relevant nanomolar fluorophore concentrations, and in particular reduces the light fluctuations present from the room lights, making low concentration measurements more reliable. This is particularly relevant for the light bands near 700nm that are more dominated by ambient lights.

Source: Biomedical Optics Express

DOI: 10.1364/boe.8.002635

Authors: Kristian J. Sexton, Yan Zhao, Scott C. Davis, Shudong Jiang, Brian W. Pogue

Institution: Dartmouth College

Language: English

Topic: Optical Imaging and Spectroscopy Techniques

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,913