1P089 Theoretical design of functionalized proteins with ideal scaffold(01F. Protein:Engineering,Poster)
Year: 2013
Type: conference-abstract
Abstract: A plant Richadella dulcifica has a glycoprotein; miraculin.This protein makes homodimer structure by forming a disulfide bond at the position of Cys138.In addition, this protein has three disulfide bonds in one subunit.Generally, when it carried out overexoression of protein using E.coli, the various problems, such as the formation of inclusion bodies, can occur.In this study, we constructed an overexpression system using E.coli BL21 with plasmid pET-16b in order to obtain the active form of the protein.As a result of expression, the protein was confirmed but it was isolated insoluble fraction for the inclusion body.We examined the various plasmid, species, strain and culture conditions.Further, the purification procedure was also examined.
Source: Seibutsu Butsuri
Institution: University of Washington
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2610315409