2P003 Conformational change of a cytoplasmic fragment of FlhA, a flagellar type III protein export apparatus protein(01A. Protein: Structure,Poster)
Year: 2013
Type: conference-abstract
Abstract: Flavoenzymes RebC and StaC are responsible for selective production of arcyriaflavin A and K252c in indolocarbazole biosynthesis, respectively.In this study, we have determined X-ray crystallographic structure of a mutant RebC F216V/R239N that has StaC-type activity at 2.4-Å resolution with reduced flavin.Compared to the putative substrate-bound WT RebC, the structural changes are confined to the active site.The side chain of Arg230, which are in between the two mutation sites, moves farther away from substrate binding site.As a result, the distance between substrate and FAD is longer than that in WT, which may not appropriate for flavin dependent monooxygenation in the mutant.
Source: Seibutsu Butsuri
Authors: Yuya Ogawa, Noritaka Hara, Yumiko Uchida, Miki Kinoshita, Tohru Minamino, Katsumi Imada
Institution: The University of Osaka
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2610534966