Year: 2009
Type: dissertation
Abstract: The Varkud Satellite (VS) ribozyme is the largest of the “small” nucleolytic \nribozymes and is the only one for which there are no high resolution crystal structures \navailable. The VS ribozyme comprises a catalytic domain and a substrate domain. The \ncatalytic domain includes five helices that interact with the stem-loop substrate. \nThe substrate is docked within a cleft that is formed by helices II and VI. This \nnaturally brings the cleavage site in close proximity to the A730 loop in helix VI. The \nadenines within the A730 loop are very crucial to the cleavage reaction and any \nsubstitution causes a major decrease in the cleavage activity of the ribozyme. \nThis study is aimed at designing and producing a variant of the Varkud Satellite \nribozyme that consists of multiple parts that can be used for detailed studies of \nribozyme kinetics and assembly.
Source: UWSpace (University of Waterloo)
Author: Allen Wing Ho Chu
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2610750201