Investigating the Conservation of Shikimate Kinase-Like 1 Function in Solanum lycopersicum and Physcomitrella patens
Year: 2015
Type: dissertation
Abstract: Investigating the Conservation of Shikimate Kinase-Like 1 Function in Solanum lycopersicum and Physcomitrella patens Masters of Science 2015 Adrian Hofmann Graduate Department of Cell and Systems Biology University of Toronto A shikimate kinase (SK) gene duplicate annotated as shikimate kinase-like 1 (SKL1) has been identified in higher plants. Physcomitrella patens is one of the most ancient land plants, and is also the most ancient SKL1 ortholog. This suggests that the SK-SKL1 gene duplication event occurred during the emergence of land plants roughly 400-500MYA. SKL1 is required for plastid biogenesis and so has neofunctionalized since the gene duplication event. However, many questions remain to be answered such as the conservation of function across land plants and the time of SKL1 neofunctionalization. In order to investigate the conservation of SKL1 function in land plants, virus induced SKL1 silencing as well as RNAi and knockout mutant generation will be employed in Solanum lycopersicum and Physcomitrella patens respectively. Additionally, experimentation using Physcomitrella patens will provide us the opportunity to investigate the time of SKL1 neofunctionalization.
Source: TSpace (University of Toronto)
Author: Adrian M. Hofmann
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2611267783