Work

2P163 Anchoring mechanism of myosin VI revealed with a programmed DNA origami spring(11. Molecular motor,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Year: 2014

Type: conference-abstract

Abstract: Myosin VI physiologically functions as transporter and anchor in a cell.Recent single molecule high accuracy nano imaging (Nishikawa et al., 2010) revealed myosin VI has inchworm-like steps and hand-over-hand steps.These diverse stepping mode may relate to the switch between transporter and anchor, however, there's no evidence for that.Here, we examined the relashionship by a direct observation of stepping dynamics under tension, because tension triggers the switching between transporter and anchor.We achieved FIONA under tension by constructing nano sized spring using DNA nanotechnology (DNA origami).We found, during anchoring, myosin VI does not only prolong dwell time but repeats inchworm-like step and back steps to keep the anchoring state.

Source: Seibutsu Butsuri

DOI: 10.2142/biophys.54.s222_1

Authors: Mitsuhiro Iwaki, Shelley Wickham, Keigo Ikezaki, Toshio Yanagida, William Shih

Institutions: RIKEN, The University of Osaka, Harvard University, The University of Tokyo

Language: English

Topic: Force Microscopy Techniques and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: diamond

OpenAlex ID: W2611857026

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