Roles of NADPH oxidase and mitochondria in flow‐induced vasodilation of human adipose arterioles: ROS ‐induced ROS release in coronary artery disease
Year: 2017
Type: article
Abstract: Objectives H2O2 contributes to FID of human arterioles. This study is designed to examine the roles of mitochondria and NADPH oxidase in modulating the release of ROS and in mediating FID. We tested whether NADPH oxidase contributes to mitochondrial ROS generation in arterioles during CAD. Methods Visceral adipose arterioles obtained from patients with or without CAD were cannulated and pressurized for videomicroscopic measurement of arteriolar diameters. Dilator responses and ROS production during flow were determined in the presence and absence of the NADPH oxidase inhibitor gp91ds‐tat and the mitochondrial electron transport inhibitor rotenone. Results Both dilation and H2O2 generation during flow were reduced in the presence of rotenone (13.5±8% vs 97±% without rotenone) or gp91ds‐tat in patients with CAD, while patients without CAD exhibited H2O2‐independent dilations. Mitochondrial superoxide production during flow was attenuated by gp91ds‐tat in arterioles from CAD patients. Conclusions These findings indicate that ROS produced by NADPH oxidase are an upstream component of the mitochondria‐dependent pathway contributing to flow‐dependent H2O2 generation and dilation in peripheral microvessels from patients with CAD. We conclude that in CAD, both mitochondria and NADPH oxidase contribute to FID through a redox mechanism in visceral arterioles.
Source: Microcirculation
DOI: 10.1111/micc.12380
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Effects of Low Carbohydrate and Low Fat Diets on Endothelium Function in Obesity, G389430949, G52457205, G6651184652, Mechanism of Flow-Induced Dilation in the Human Microcirculation, Mechanisms of exertional hypertension and vascular dysfunction, Obesity and Endothelial Dysfunction: Kir channels in the progression of vascular disease, Improving Blood Pressure & Vascular risk with resistance exercise in African Americans
OpenAlex ID: W2612712906