Year: 2017
Type: article
Abstract: Efforts to manipulate locus-specific histone acetylation to assess their causal role in gene expression and cellular and behavioural phenotypes have been impeded by a lack of experimental tools. The Cas9 nuclease has been adapted to target epigenomic modifications, but a detailed description of the parameters of such synthetic epigenome remodellers is still lacking. Here we describe a Cas9-based histone deacetylase (HDAC) and the design principles required to achieve locus-specific histone deacetylation. We assess its range of activity and specificity, and analyse target gene expression in two different cell types to investigate cellular context-dependent effects. Our findings demonstrate that the chromatin environment is an important element to consider when utilizing this synthetic HDAC.
Source: Nature Communications
DOI: 10.1038/ncomms15315
Institution: University of Pennsylvania
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Awards: Understanding the Epigenetic Mechanisms Underlying Stress-Related Neuropsychiatric Disorders, Understanding the Epigenetic Mechanisms Underlying Stress-related Neuropsychiatric Disorders, Defining the Epigenetic Architecture Associated with Early-Life Stress, Training Program in Neurodevelopmental Disabilities
OpenAlex ID: W2612830428