Year: 2016
Type: article
Abstract: Introduction: Thicker ciliary muscles have been associated with more myopic refractive errors.The mechanism leading to thicker ciliary muscles in myopic subjects is currently unknown.Therefore, the purpose of this dissertation was to develop methods for understanding the ciliary muscle and to begin to dissect the mechanism(s) that could be leading thicker ciliary muscles in myopic subjects.Methods: Guinea pigs were the primary subjects utilized in this study.Retinoscopy, ultrasound, and photometric analysis were used to understand biometric outcomes (e.g., refractive error, axial length).Histology was used to analyze and track normal guinea pig ciliary muscle development and differences between form-deprived and control eyes.Uniaxial stretch was applied to guinea pig eyes, and control and treated eyes were compared with RNA-Seq.RNA-Seq was also used to determine differences between form-deprived and control eyes.Results: Normal ciliary muscle volume was significantly correlated with log age (p = 0.001), ocular length (p = 0.003), limbal circumference (p = 0.01), and equatorial diameter (p = 0.003), though it was not related to normal refractive error.Normal ciliary muscle growth occurs via a combination of hypotrophy and hyperplasia during the first 20 days of life, and it switches to pure hypertrophy thereafter.Stretch induced ocular genetic changes Dedication This document is dedicated to my wife Emily and supportive family.
Author: Andrew D. Pucker
Institution: The Ohio State University
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2612979226