Identification of factors required for m 6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI
Year: 2017
Type: article
Abstract: Summary N 6‐adenosine methylation (m 6 A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m 6 A writer proteins in Arabidopsis thaliana . The components required for m 6 A in Arabidopsis included MTA , MTB , FIP 37, VIRILIZER and the E3 ubiquitin ligase HAKAI . Downregulation of these proteins led to reduced relative m 6 A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m 6 A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m 6 A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.
Source: New Phytologist
DOI: 10.1111/nph.14586
Institutions: Central European Institute of Technology, University of Helsinki, Masaryk University, Central European Institute of Technology – Masaryk University, University of Nottingham, Universidade do Porto, Centro de Investigação em Biodiversidade e Recursos Genéticos, Ghent University, VIB-UGent Center for Plant Systems Biology, Chinese University of Hong Kong, University of Cambridge, Sainsbury Laboratory
Language: English
Topic: RNA modifications and cancer
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,790
Funders: National Science Foundation, Helsingin Yliopisto, Ministerstvo Školství, Mládeže a Tělovýchovy, Grantová Agentura České Republiky, Academy of Finland, Tekes, Directorate for Biological Sciences, Biotechnology and Biological Sciences Research Council, European Social Fund, H2020 European Research Council
Awards: G1179740972, G1432604747, Development of diagnostic tools for detection and quantifications of mRNA methylation, G272274071, Defining the plant epitranscriptome, G2868429769, G3262385548, G3287583965, G3761558573, Role of RNA splicing in protoxylem cell fate, G428489134, G4797037343, CEITEC 2020, G5208924892, 14 NSFBIO: Bilateral NSF/BIO-BBSRC - Modeling the effects of intrinsic and extrinsic signaling on cellular differentiation in plants, G5856400019, G620827937, National Infrastructure for Biological and Medical Imaging, National Center for Medical Genomic, G685278153, The Arabidopsis Epitranscriptome, G9000262362
OpenAlex ID: W2613267149