Year: 2017
Type: dissertation
Abstract: In this thesis, the use of quantitative ultrasound techniques is expanded from previous studies to quantify the approximate degree of tumor response with other modalities of cell death while determining nuclear chromatin to be the major scatterer of ultrasound at frequencies around 20-40 MHz. In the first study, quantitative ultrasound parameters correlated strongly to tumor cell death following mitotic arrest on the basis of chromatin aggregate formation. In the second study, it was observed that quantitative ultrasound techniques could differentiate cell death modalities in a manner that was linked to different chromatin manners of chromatin processing within each form of cell death. In the final study, quantitative ultrasound parameter trends mirrored those of chromatin compaction in the course of chromatin-altering treatments. The findings of this work indicate that quantitative ultrasound can characterize and differentiate multiple forms of cell death in a quantitative manner based on changes to chromatin configuration.
Source: TSpace (University of Toronto)
Author: Maurice Pasternak
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2614014622