Year: 2016
Type: dissertation
Abstract: The study of plant mineral nutrition and toxicity has made major strides recently, particularly at the level of molecular genetics. Arguably, however, this has been at the expense of “classical” physiology, which is of concern because critical physiological examinations of cellular and molecular models in planta are needed if extrapolations of these models to “real-world” (field-level) conditions are to be made. This is particularly urgent in the face of increasing environmental degradation and global food demands. To this end, the preset work explores the physiological role of membrane channels in higher-plant nutrition and toxicology, building upon foundational work in nutritional physiology and applying the important new discoveries in the areas of molecular-genetics and electrophysiology. Combining radioisotopic (42K+ and 13NH3/13NH4+) flux kinetics and compartmentation analyses with techniques in electrophysiology, mutant analysis, gas exchange, fluorescence imaging and tracing, and tissue mineral-content analysis, this work investigates the involvement of inward- and outward-rectifying Shaker-like K+ channels (KIR and KOR, respectively), nonselective cation channels (NSCCs), and aquaporins (AQPs), in K+ and NH3/NH4+ transport under conditions of both stress (low K+, salinity, and ammonium toxicity) and non-stress, in the intact plant. Efflux analyses showed that KOR channels mediate K+ efflux in barley roots only at low external [K+] (
Source: TSpace (University of Toronto)
Author: Devrim Coskun
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2614081479