Year: 1997
Type: dissertation
Abstract: cette thèse sous paper or electronic formats.la forme de microfiche/nlm, de reproduction sur papier ou sur format électronique.The author retains ownership of the L'auteur conserve la propriété du copyright in this thesis.Neither the droit d'auteur qui protège cette thèse.thesis nor substantial extracts fiom it Ni la thèse ni des extraits substantiels may be printed or othemise de celle-ci ne doivent être imprimés reproduced without the author's ou autrement reproduits sans son permission.autorisation.2-1 1. Nothern blot of rainbow trout total RNA from different developmental stages 2-1 S. In situ hybridization on rainbow trout embryos witb rtALB2 cDNA 77 2-13.Different levels of the rtALB 1 and rtALB2 mRNA 2-14.The probes used in nuclear run-on experiment 2-15.Cornparison of the transcriptional rates of nALB 1 and rtALB2 gene by nuclear run-on assay 8 1 Two hypothesis of rainbow mut albumin gene evolution Smtegy of resaiction mapping of genomic DNA clones Restriction maps of genomic clone 4 and clone 16 Subclone strateg for clone g16-5 Nested deletions of clone g 16-5H2kSwtegy for rainbow trout aibuminî promoter-CAT construction IO3 Southern analysis of positive genomic clones for trout albumin gene Resrriction rnapping of genomic clones g4 and g16 ?CR searching the location of the nanscnption stan site Partial DNA sequence of the rtALB2 gene Primer extention mapping of the mscripaon stan site Southem hybridization of genomic DNA with rtALB2 cDKA probe Southem hybridization of genomic DN.4 with the nALB2 5' flanking probe 128 Potential DNA binding sites for some transcription factors in the 5' flanking region oFnALB2 gene CAT activities of albumin-CAT constmcts 132 PCR cloning of the nALB 1 promoter 134 3-1 6. Cornparison of albumin promoten 3-17.Functiond cornparison of the two rainbow uout albumin gene promoters 139 3-1 8. Site-directed mutations on the r ~4 L B 2 promoter 1 40 3-19.CATactivitiesof mutants List of Tables 1 -1.A N n o acid composition of serum albumin 4 2-1.Cornparison of silent mutation and replacement muration of some genes in salmon 85 3-1.Sumrnary of southern results of rainbow trout aibumin genomic clones 109 3-2.Cornparison of cis elements in proximal prornoter regions of ALB, AFP. and DBP genes v i i Acknow ledgments 1 would like to thank my supervisor Dr. Choy L. Hew for giving me the opponunity to work in his laboratos., and for al1 his guidance and suppon over the years.I wish to especially thank him for his encouragement during difficult tirnes to make
Author: Zhi Gong
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2614337752