Year: 2016
Type: dissertation
Abstract: Poly(methacrylic acid-co-methyl methacrylate) (MAA) beads improve vascularization when applied to cutaneously-wounded diabetic mice. The aim of this thesis is to understand the vascular regenerative properties of MAA at the cellular and molecular level. Subcutaneous injection of MAA beads promoted the formation of a denser and perfusable network of blood vessels at days 3 and 7 relative to poly(methyl methacrylate) (MM) control beads. MAA beads modulated the host response; promoting more neutrophils at day 1 and more macrophages at day 7, relative to MM beads. A M2 macrophage polarization bias was observed in MAA-treated animals but not in MM-treated animals. Additionally, complement was involved in the mechanism of MAA; complement inhibition (at the C1 or C3 levels) diminished the M2 polarization bias at day 3, although no changes in vascularity were noted. These findings deepen our understanding of MAA and benefit the development of MAA-based biomaterials for\napplications in regenerative medicine.
Source: TSpace (University of Toronto)
Author: Kongyu David Zhang
Language: English
Topic: Wound Healing and Treatments
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2614482634