Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast
Year: 2017
Type: article
Abstract: Bipolar spindle assembly requires a balance of forces where kinesin-5 produces outward pushing forces to antagonize the inward pulling forces from kinesin-14 or dynein. Accordingly, Kinesin-5 inactivation results in force imbalance leading to monopolar spindle and chromosome segregation failure. In fission yeast, force balance is restored when both kinesin-5 Cut7 and kinesin-14 Pkl1 are deleted, restoring spindle bipolarity. Here we show that the cut7Δpkl1Δ spindle is fully competent for chromosome segregation independently of motor activity, except for kinesin-6 Klp9, which is required for anaphase spindle elongation. We demonstrate that cut7Δpkl1Δ spindle bipolarity requires the microtubule antiparallel bundler PRC1/Ase1 to recruit CLASP/Cls1 to stabilize microtubules. Brownian dynamics-kinetic Monte Carlo simulations show that Ase1 and Cls1 activity are sufficient for initial bipolar spindle formation. We conclude that pushing forces generated by microtubule polymerization are sufficient to promote spindle pole separation and the assembly of bipolar spindle in the absence of molecular motors.
Source: Nature Communications
DOI: 10.1038/ncomms15286
Authors: Sergio A. Rincon, Adam Lamson, Robert Blackwell, Viktoriya Syrovatkina, Vincent Fraisier, Anne Paoletti, Meredith D. Betterton, Phong T. Tran
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: National Science Foundation, Infrastructures en Biologie Santé et Agronomie, Agence Nationale de la Recherche, Université de Lausanne, National Institutes of Health, University of Colorado Boulder, University of California, San Francisco, National Institute of General Medical Sciences, Division of Materials Research
Awards: A High-memory Supercomputer for Proteomics, Text Mining and Microbiome Research, G1604523534, G1821513860, Paris Sciences et Lettres, G2833630727, G3025942069, Understanding mitotic spindle positioning by integrated modeling and experiment, G4705984246, Soft Materials Research Center, Des cellules aux tissus: au croisement de la Physique et de la Biologie, Mechanisms of spindle formation, G6746967076, G7749454669, EAGER: Biophysical Theory of Mitotic Spindle Length Instability and Self Assembly, G9000072324, G9000187623
OpenAlex ID: W2615947293