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Extracellular Phosphodiesterase from the Growth Medium of the Myxomycete Physarum flavicomum

Year: 1984

Type: article

Abstract: The plasmodium of the myxomycete Physarum flavicomum secretes cyclic AMP phosphodiesterase into the medium. The extracellular enzyme had a pH optimum between 7 and 8 and a Km of about 500 μM cyclic AMP and was inhibited by theophylline, caffeine and 3-isobutyl-l-methyxanthine (MIX). A marked decrease of enzyme activity was noted in the presence of EDTA, suggesting the requirement of Mg+ + by the enzyme. Addition of Mg+ + and Ca + + stimulated the enzyme while Zn+ + , Co+ + , Pb+ + , Mn+ + , Fe + + + , Ni+ + , and Cu + + all inhibited phosphodiesterase activity. An interesting feature of this extracellular phosphodiesterase was its ability to retain full catalytic activity after prolonged exposure to elevated temperatures

Source: Journal of the Arkansas Academy of Science

Authors: Thomas J. Lynch, Mary E. Farrell

Language: English

Topic: Phosphodiesterase function and regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, Office of Experimental Program to Stimulate Competitive Research

OpenAlex ID: W2616409554

FWCI: 0.143

Cites:

Cited by: 1

Related to: 20