Relationship of mechanical impact magnitude to neurologic dysfunction severity in a rat traumatic brain injury model
Year: 2017
Type: article
Abstract: OBJECTIVE: Traumatic brain injury (TBI) is a major brain injury type commonly caused by traffic accidents, falls, violence, or sports injuries. To obtain mechanistic insights about TBI, experimental animal models such as weight-drop-induced TBI in rats have been developed to mimic closed-head injury in humans. However, the relationship between the mechanical impact level and neurological severity following weight-drop-induced TBI remains uncertain. In this study, we comprehensively investigated the relationship between physical impact and graded severity at various weight-drop heights. APPROACH: The acceleration, impact force, and displacement during the impact were accurately measured using an accelerometer, a pressure sensor, and a high-speed camera, respectively. In addition, the longitudinal changes in neurological deficits and balance function were investigated at 1, 4, and 7 days post TBI lesion. The inflammatory expression markers tested by Western blot analysis, including glial fibrillary acidic protein, beta-amyloid precursor protein, and bone marrow tyrosine kinase gene in chromosome X, in the frontal cortex, hippocampus, and corpus callosum were investigated at 1 and 7 days post-lesion. RESULTS: Gradations in impact pressure produced progressive degrees of injury severity in the neurological score and balance function. Western blot analysis demonstrated that all inflammatory expression markers were increased at 1 and 7 days post-impact injury when compared to the sham control rats. The severity of neurologic dysfunction and induction in inflammatory markers strongly correlated with the graded mechanical impact levels. CONCLUSIONS: We conclude that the weight-drop-induced TBI model can produce graded brain injury and induction of neurobehavioral deficits and may have translational relevance to developing therapeutic strategies for TBI.
Source: PLoS ONE
Language: English
Subfield: Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: G1052468712, Effects of Central Neuromodulation with Functional Electrical Stimulation on Bladder Functions in Traumatic Brain Injured Rats, G329607045, G456520585, G4940008598, Development of Electrophysiological Biomarkers and Their Therapeutic Cortical Electrical Stimulation for Improving Neuroplasticity and Motor Function in Parkinsonian Rats, G687344902, G7607085911, Mapping progressive loss of intracortical inhibition by TMS and EEG in posttraumatic epileptogenesis, The Development and Clinical Application of a Miniature Transcranial Direct Current Stimulator in Rehabilitation Therapy (II), G9000028250, G951433114
OpenAlex ID: W2618096553