Year: 2014
Type: article
Abstract: function in Ppp1cc -/-mice.We generated four different transgenic Rescue mice lines.The first three transgenic lines were generated using cDNA of PP1γ1 mRNA including the initial sequence or entire intron 7. Lack of fertility in these three lines was attributed to low levels of transgenic PP1γ1 in testis probably due to instability of the PP1γ1 mRNA containing intron 7 in testis.Testis expression of PP1γ1 occurred at significant levels only when the transgene lacked the intron7.Levels of the PP1γ1 transgene in this line (Rescue IV) were comparable to PP1γ2 levels in Ppp1cc +/-mice testes.Sperm morphogenesis was restored with the PP1γ1 transgene in Ppp1cc -/-mice but small percentage of these males remained infertile due to altered sperm motility.The findings of this study suggests that despite the ability of PP1γ1 to support sperm morphogenesis, it is detrimental for male fertility and hence excluded in differentiating spermatogenic cells by alternate splicing and miRNA mediated instability of its transcript.
Author: Tejasvi Dudiki
Institution: Kent State University
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2619594766