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Efficient Transduction of Zebrafish Melanoma Cell Lines and Embryos Using Lentiviral Vectors

Year: 2017
Type: article
Abstract: The establishment of in vitro cultures of zebrafish cancer cells has expanded the potential of zebrafish as a disease model. However, the lack of effective methods for gene delivery and genetic manipulation has limited the experimental applications of these cultures. To overcome this barrier, we tested and optimized vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped lentiviral and retroviral vector transduction protocols. We show that lentivirus successfully and efficiently transduced zebrafish melanoma cell lines in vitro, allowing antibiotic selection, fluorescence-based sorting, and in vivo allotransplantation. In addition, injection of concentrated lentiviral particles into embryos and tumors established the feasibility of in vivo gene delivery.
Source: Zebrafish
DOI: 10.1089/zeb.2017.1434
Authors: Maurizio Fazio, Serine Avagyan, Ellen van Rooijen, William Mannherz, Charles K. Kaufman, Riadh Lobbardi, David M. Langenau, Leonard I. Zon
Institutions: Boston Children's Hospital, Dana-Farber Cancer Institute, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Washington University in St. Louis, Massachusetts General Hospital, Howard Hughes Medical Institute, Harvard University, Harvard Stem Cell Institute
Language: English
Subfield: Genetics
Open Access status: green
OpenAlex ID: W2619684752
FWCI: 0.181
Cites: 12
Cited by: 4
Related to: 10