RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes
Year: 2015
Type: article
Abstract: Increasing rates of obesity and associated complications worldwide have increased research interest in the complex interplay of hormones, metabolism, and adiposity.There is much evidence indicating that hormones such as growth hormone (GH), insulin-like growth factor 1 (IGF-1), and insulin affect white adipose tissue (WAT) in a depot-specific manner.This suggests that hormone-induced intracellular signaling is more active in some WAT depots compared to others, as evidenced by hormone receptor expression levels.To add another layer of complexity, WAT is made up of many cell types with distinct functions, including adipocytes and cells of the stromal vascular fraction (SVF), which can differentially express these receptors.The purpose of this thesis is to further develop our understanding of how GH-, IGF-1-, and insulin-induced signaling affect adiposity in a WAT depot-specific manner.The original aims of this thesis were to: 1) use quantitative polymerase chain reaction (qPCR) to compare the expression of the receptors of the above hormones (GH, IGF-1, and insulin) in the SVF and adipocyte portions of the most commonly studied WAT depots in wildtype (WT) mice (subcutaneous, epididymal, retroperitoneal, and mesenteric), and 2) compare the expression of these receptors in the WAT depots of growth hormone antagonist (GHA) and WT mice.Unfortunately, due to low adipocyte RNA concentrations and purity, the first aim was adjusted to examine receptor RNA expression in whole WAT (as opposed
Author: Vivian A. Lesende
Institution: Ohio University
Language: English
Subfield: Physiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2621091664