Testing the Role of an Arf GTPase-activating Protein dASAP in Epithelial Cell Polarity in the Drosophila Embryo
Year: 2011
Type: dissertation
Abstract: Baz/PAR3 is a key regulator of epithelial cell polarity (ECP). To identify proteins functioning with Baz, I completed a baz genetic interaction screen by localizing 15 GFP-tagged candidates. Then I tested the role of a top candidate, dASAP (Drosophila Arf GTPase-activating protein with SH3 domain, Ankyrin repeat and PH domain), in Drosophila ECP. To determine whether dASAP might interact with polarity players, I defined the localization of dASAP throughout embryogenesis with GFP-tagged proteins and an anti-dASAP antibody. To study how loss of dASAP function affects ECP, I generated a deletion allele by imprecise P-element excision. To evaluate how each of the six domains of dASAP contributes to its localization and functions, I generated constructs deleting each domain. I found associations between dASAP, actin and the apical domain. The six domains may act redundantly to localize dASAP, although interactions between domains may affect the degree of membrane association.
Source: TSpace (University of Toronto)
Author: Wei Shao
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Award: G3913132647
OpenAlex ID: W2623857709