This work has been retracted.
RETRACTED ARTICLE: Brain-derived exosomes from dementia with Lewy bodies propagate α-synuclein pathology
Year: 2017
Type: article
Abstract: Proteins implicated in neurodegenerative conditions such as Alzheimer’s disease (AD) and Dementia with Lewy Bodies (DLB) have been identified in bodily fluids encased in extracellular vesicles called exosomes. Whether exosomes found in DLB patients can transmit pathology is not clear. In this study, exosomes were successfully harvested through ultracentrifugation from brain tissue from DLB and AD patients as well as non-diseased brain tissue. Exosomes extracted from brains diagnosed with either AD or DLB contained aggregate-prone proteins. Furthermore, injection of brain-derived exosomes from DLB patients into the brains of wild type mice induced α-synuclein (α-syn) aggregation. As assessed through immunofluorescent double labeling, α-syn aggregation was observed in MAP2 + , Rab5 + neurons. Using a neuronal cell line, we also identified intracellular α-syn aggregation mediated by exosomes is dependent on recipient cell endocytosis. Together, these data suggest that exosomes from DLB patients are sufficient for seeding and propagating α-syn aggregation in vivo.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,790
Awards: G1311847726, G2739352285, a-synuclein clearance strategies for the treatment of Lewy body disease, G2975746713, a-Synuclein vulnerability mechanisms and therapeutics in Alzheimer's Disease, G3192769674, UCSD Alzheimer's Disease Research Center P50, Pathogenicity of neuronally-derived tau in exosomes, A Proinflammatory Endophenotype to Predict NSAID Treatment Response Alzheimer's Disease Clinical Trials, Training in the Neuroplasticity of Aging, G7733295757, G8403866244, G9000142624, G9000303919
OpenAlex ID: W2624248893