BRUTUS and its paralogs, BTS LIKE1 and BTS LIKE2, encode important negative regulators of the iron deficiency response in Arabidopsis thaliana
Year: 2017
Type: article
Abstract: Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe-deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions.
Source: Metallomics
DOI: 10.1039/c7mt00152e
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Funders: National Science Foundation, U.S. Department of Energy, National Institutes of Health, Office of Science, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Environmental Health Sciences, Basic Energy Sciences, Biological and Environmental Research, Brookhaven National Laboratory, SLAC National Accelerator Laboratory
Awards: DEVELOPMENT OF SYNCHROTRON X-RAY MICRO-SPECTROSCOPIC TECHNIQUES AND APPLICATION TO PROBLEMS IN LOW TEMPERATURE GEOCHEMISTRY, DEVELOPMENT OF SYNCHROTRON X-RAY MICRO-SPECTROSCOPIC TECHNIQUES AND APPLICATION TO PROBLEMS IN LOW TEMPERATURE GEOCHEMISTRY, G1126863173, G1229526393, G1286251239, G132563423, G1336448005, G1572106353, SYNCHROTRON X-RAY MICROPROBE AND MICROSPECTROSCOPY RESEARCH IN LOW TEMPERATURE GEOCHEMISTRY, G1804828992, G1831385526, G222768804, G2371548206, G2393997808, G262532246, Sources and Protracted Effects of Early Life Exposure to Arsenic and Mercury, G303061748, G315621713, G3243759740, G338944111, G3488436650, G3629260272, G4235786860, G4280418065, G4581610328, G4654690619, G4768711226, G495301720, G498139845, G5094664921, G5292656900, G549834383, G5570207115, G5648016710, G6550344761, The Genetic basis of Natural Ionomic Variation, G7283024673, G8030439883, G8382000481, G8435008881, G8507651203, A Synchrotron Radiation Structural Biology Resource, G8925327636, Cancer Center Support Grant, G911061179, G969889393
OpenAlex ID: W2626126515