The impact of inflammatory cytokines, il-6 and il-1beta, on the pathogenesis of immune failure in HIV disease
Year: 2014
Type: article
Study design: Observational Study
Abstract: Study (CLIF) 4 Inflammatory cytokines IL-1β and IL-6 7Interleukin-1β 9 Interleukin-6 11Induction of IL-6 in HIV disease 13 Gut barrier integrity breach and microbial translocation in HIV disease 15The role of IL-7 in T cell homeostasis and HIV disease 17 The anti-apoptotic factor B-cell lymphoma 2 (Bcl2) 18 Lymph node fibrosis 20 Cellular Senescence 21 Chapter 2: The Cleveland Immune Failure study (CLIF): Failure to 25 recover CD4 T cells is associated with immune senescence and discordance between plasma IL-7 levels and IL-7 responsiveness v Abstract Introduction Methods Patients Cytokines Cell Culture Flow Cytometry Statistics Results Immune senescence marker, PD-1 and CD57, are elevated on CD4 T cells in immune failure patients Plasma levels of IP-10 are elevated in immune failure patients and correlate with indices of immune activation and inflammation Plasma levels of IL-7 are elevated in immune failure patients but do not correlate with CD4 T cell cycling or naïve CD4 T cell numbers Intestinal fatty acid binding protein (I-FABP) levels are elevated in the plasma from both patient groups however, no significant difference were seen in plasma levels of I-FABP, LPS, or zonulin between immune failure patients and immune success patients Discussion Chapter 3: HIV is not a major driver of increased plasma IL-6 levels in chronic HIV disease Abstract Introduction Methods Patients vi Virus preparation and storage HIV infection of macrophages Cytokine production Colonic mucosal histoculture HIV P24 measurement Lymph node histoculture Statistics Results Plasma IL-6 levels correlate weakly with plasma HIV RNA levels before administration of antiretroviral therapies.HIV infection of monocyte-derived macrophages does not induce IL-6 production HIV infection of colonic histocultures does not induce IL-6 production Lymph node histocultures infected with HIV in vivo or ex vivo do not increase IL-6 production Discussion Chapter 4: Inflammatory cytokines drive CD4 T cell cycling and impaired responsiveness to interleukin-7: Implications for immune failure in HIV disease
Author: Carey L. Shive
Institution: Case Western Reserve University
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2626244038