Recent advances in functional perturbation and genome editing techniques in studying sea urchin development
Year: 2017
Type: review
Abstract: Studies on the gene regulatory networks (GRNs) of sea urchin embryos have provided a basic understanding of the molecular mechanisms controlling animal development. The causal links in GRNs have been verified experimentally through perturbation of gene functions. Microinjection of antisense morpholino oligonucleotides (MOs) into the egg is the most widely used approach for gene knockdown in sea urchin embryos. The modification of MOs into a membrane-permeable form (vivo-MOs) has allowed gene knockdown at later developmental stages. Recent advances in genome editing tools, such as zinc-finger nucleases, transcription activator-like effector-based nucleases and the clustered regularly interspaced short palindromic repeat/clustered regularly interspaced short palindromic repeat-associated protein 9 (CRISPR/Cas9) system, have provided methods for gene knockout in sea urchins. Here, we review the use of vivo-MOs and genome editing tools in sea urchin studies since the publication of its genome in 2006. Various applications of the CRISPR/Cas9 system and their potential in studying sea urchin development are also discussed. These new tools will provide more sophisticated experimental methods for studying sea urchin development.
Source: Briefings in Functional Genomics
DOI: 10.1093/bfgp/elx011
Authors: Miao Cui, Che-Yi Lin, Yi-Hsien Su
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Life below water
Open Access status: bronze
Awards: Integration of Advanced Biotechnology and Conventional Breeding Technology in Improving Crop Stress Tolerance and Productivity( II ), Integration and Evolution of Hypoxia Response Network: Systems Biology and Evolution Approach (III), Molecular Mechanisms for Symmetry Breaking and Establishing Left-Right Asymmetry during Embryogenesis, G6834145290
OpenAlex ID: W2626683726