Year: 2015
Type: dissertation
Abstract: The purpose of this study was to identify the oral microbiome in siblings with or without dental caries using next generation sequencing to verify the ecological changes in health and caries state and to identify the horizontal transmission. To investigate the composition of microbiome, 14 young siblings including 7 in caries group and 7 in caries-free group (average of 4.3 years) were enrolled in 7 families. Supragingival plaques were collected from cervico-buccal area of posterior teeth. All samples were analyzed by pyrosequencing based on 16S rRNA gene V1-V4 hypervariable regions. At the phylum level of caries-group, Firmicutes increased and Proteobacteria, Bacteroidetes, Fusobacteria, and Actinobacteria decreased. At the genus level of caries-group, Streptococcus increased and Haemophilus, Capnocytophage, and Leptotrichia decreased. At species level of caries group, Veillonella dispar, Veillonella tobetsuensis, Veillonella rodentium, Actinomyces viscosus, and Streptococcus sanguinis increased and Streptococcus mitis, Capnocytophage sputiger, Capnocytophage leadbeltt and AF366267 decreased. Similarity between the siblings was evident in UniFrac distance (p<0.05). In conclusion, these results showed the ecological change of oral microbiome in caries, the same as the previous researches. In addition, it appeared that the horizontal transmission contributed to the colonization of oral microbiome in siblings. We suggested that the strategy of caries prevention and management have to be considered in more holistic and personalized approach.
Author: 이효설
Language: English
Subfield: Periodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2627075584